Sulforaphane Upregulates Cultured Mouse Astrocytic Aquaporin-4 Expression through p38 MAPK Pathway

Figure 3

Effect of SFN on apoptosis of cultured mouse astrocytes. (a) TUNEL assay showed no significant difference in cell apoptosis among astrocytes treated with 0, 1, and 5 μM SFN for 24 hours. (b–e) Flow cytometry showed no significant difference in normal cells (Q4), early apoptotic cells (Q3), and late apoptotic cells (Q2) among astrocytes treated with 0, 1, and 5 μM SFN for 24 hours. Q1:(Annexin V-FITC)-/PI+; Q2: (Annexin V + FITC)+/PI+; Q3: (Annexin V-FITC)+/PI-; Q4: (Annexin V-FITC)-/PI-. (f) Western blotting testing the activation of Caspase-3. The result showed that no cleaved Caspase-3 fragments (17 KDa and 19 KDa molecular weight) were detected among astrocytes treated with 0, 1, and 5 μM SFN for 24 hours.

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