Sulforaphane Upregulates Cultured Mouse Astrocytic Aquaporin-4 Expression through p38 MAPK Pathway

Figure 5

Effect of SFN on AQP4 expression and phosphorylation of p38 MAPK of cultured mouse astrocytes by WB. (a) Effect of SFN on AQP4 expression and phosphorylation of p38 MAPK of cultured mouse astrocytes by WB. (b, c) Quantitative analysis for AQP4 expression (b) and phosphorylation of p38 MAPK (c). Western blotting showed significant increase in AQP4 expression in groups treated with 0.5 and 1 μM SFN for 24 hours compared to the vehicle treatment group, while the groups treated with 5 μM SFN showed significantly decreased AQP4 expression. Meanwhile, western blotting showed significant increase in phosphorylation of p38 MAPK in groups treated with 0.5, 1, and 2.5 μM SFN for 24 hours compared to the vehicle treatment group, while the groups treated with 5 μM SFN showed significantly decreased phosphorylation of p38 MAPK. Compared to the control group, .

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