Hybrid Metabolic Activity-Related Prognostic Model and Its Effect on Tumor in Renal Cell Carcinoma

Figure 7

The validation of the expression of differentially expressed genes related to the hybrid metabolic activity and the effect of the DLD gene knockdown on the cell function in RCC. (a) The relative expression levels of DLD, ALDH6A1, and SLC25A4 genes in HK-2, Caki-1, Caki-2, and 786-O cell lines were detected by the qRT-PCR. (b) The expression levels of DLD gene encoded protein in HK-2, Caki-1, Caki-2, and 786-O cell lines were detected by the western blot. (c) The relative expression levels of DLD gene detected by the qRT-PCR in ten pairs of paired RCC tissues and adjacent tissues. (d) The expression level of DLD gene in 786-0 and Caki-1 cell lines was knocked down by siRNA, and the knockdown efficiency was verified by the qRT-PCR. (e) The scratch wound-healing assay revealed that DLD knockdown inhibited the migration of tumor cells of RCC. The images were taken at 20x. (f) The Transwell assay in 786-O and Caki-1 cell lines further demonstrated that DLD knockdown inhibited the migration of tumor cells. The images were taken at 20x. (, , , ).

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