Research Article
Astrocytes Protect Human Brain Microvascular Endothelial Cells from Hypoxia Injury by Regulating VEGF Expression
Figure 4
HA-1800 protects hBMEC by VEGF. (a)-(b) Western blot to detect the transfection efficiency of knockout VEGF in HA-1800 cells. (c) The effect of VEGF knockout in HA-1800 cells on hBMEC proliferation activity under hypoxia detected by CCK-8. (d) To detect the effect of VEGF knockout on hBMEC apoptosis in HA-1800 cells under hypoxia through TUNEL. (e)-(f) To detect the effect of VEGF knockout in HA-1800 cells on SOD in hBMEC cell lysates under hypoxia.
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