[Retracted] circRNA LDLRAD3 Enhances the Malignant Behaviors of NSCLC Cells via the miR-20a-5p-SLC7A5 Axis Activating the mTORC1 Signaling Pathway

Figure 2

LDLRAD3 sponged miR-20a-5p to promote SLC7A5 expression. (a) The expression level of SLC7A5 mRNA was analyzed by qRT-PCR. (b) The expression level of SLC7A5 proteins was analyzed by western blot. (c) The expression level of SLC7A5 proteins was analyzed by immunohistochemistry. Scale bars: 50 μm. (d) The correlation between the expression levels of miR-20a-5p and SLC7A5 in NSCLC tissues was analyzed by Spearman’s correlation test. (e) The correlation between the expression levels of LDLRAD3 and SLC7A5 in NSCLC tissues was analyzed by Spearman’s correlation test. (f) The binding site of miR-20a-5p on SLC7A5 was predicted by ENCORI. (g) The binding relationship between miR-20a-5p and SLC7A5 was verified by the DLR assay. (h) The expression level of miR-20a-5p in transfected A549 and H1299 cell lines was analyzed by qRT-PCR. (i) The expression level of SLC7A5 mRNA in cotransfected cells was analyzed by qRT-PCR. (j) The expression level of SLC7A5 protein in cotransfected cells was analyzed by western blot. (k) The expression level of SLC7A5 protein in cotransfected cells was analyzed by immunofluorescent assay. Scale bars: 50 μm. and .