[Retracted] Inhibition of microRNA-665 Alleviates Septic Acute Kidney Injury by Targeting Bcl-2

Figure 2

Down-regulation of miR-665 inhibited LPS-induced inflammation and apoptosis of HK-2 cells. ((a)∼(c)) The levels of inflammatory factors (TNF-α, IL-1β, IL-6) were detected using commercial kits (“” vs. control, “#” vs. LPS + NC, n = 3). (d) The expression of TNF-α, IL-6, IL-1β, Bax and Bcl-2 was detected using Western blot (“” vs. control, “#” vs. LPS + NC, n = 3). (e) Cell viability was tested by MTT assay (“” vs. control, “#” vs. LPS + NC, n = 3). (f) Apoptosis rate was detected by flow cytometry (“” vs. control, “#” vs. LPS + NC, n = 3). (g) Apoptosis was observed by TUNEL staining (“” vs. control, “#” vs. LPS + NC, n = 3).

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