CPE Regulates Proliferation and Apoptosis of Primary Myocardial Cells Mediated by Ischemia and Hypoxia Injury

Figure 2

Overexpression of CPE can inhibit I/H-induced apoptosis of primary cardiomyocytes. (a) CPE mRNA expression in primary cardiomyocytes (control and I/H groups). , compared with control group. (b) CPE mRNA expression in primary cardiomyocytes. (c) TUNEL staining and quantitative analysis of TUNEL-positive cells. (d) Annexin V-PI staining detection for primary myocardial cell apoptosis. (e) CCK-8 assay for the primary myocardial cell activity. (f) Caspase-3 kit to detect the content of Caspase-3 in the supernatant of primary myocardial cells. (g) Caspase-9 kit to detect the content of Caspase-9 in the supernatant of primary myocardial cells. (h) Bax mRNA expression in primary cardiomyocytes. (i) Bcl-2 mRNA expression in primary cardiomyocytes. (Control, I/H, I/H + pcDNA3.1-CPE, and I/H + pcDNA3.1 groups). , compared with control group; , compared with I/H group.