Research Article
MiR-98 Protects Nucleus Pulposus Cells against Apoptosis by Targeting TRAIL in Cervical Intervertebral Disc Degeneration
Figure 4
miR-98 directly targeted 3′-UTR of TRAIL. (a) The 3′UTR of TRAIL predicted as a miR-98 target using ENCORI. (b) Luciferase activity of a reporter containing a wild-type (WT) TRAIL 3′UTR or a mutant (MUT) TRAIL 3′UTR. (c)–(e) The expression level of TRAIL after different treatments (blank, LPS, LPS + mimics-NC, and LPS + miR-98 mimics) determined by qRT-PCR, Western blotting, and immunofluorescence assay. and . Scale bars: 50 μm.
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