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Developmental Immunology
Volume 1, Issue 2, Pages 127-135

Ontogenic Appearance of MHC Class I (B-F) Antigens During Chicken Embryogenesis

1Basel Institute for Immunology, Grenzacherstrasse 487, Basel CH-4058, Switzerland
2Unstitute for Experimental Immunology, University of Copenhagen, Nørre Alle 71, Copenhagen ∅ DK-2100, Denmark

Received 13 June 1990; Accepted 19 July 1990

Copyright © 1990 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Expression of chicken MHC class (B-F) antigens during ontogeny was determined by binding of anticlass antibody and appearance of B-F transcripts by Northern blotting in chicken organs during embryogenesis until 2 weeks after hatching. MHC class transcripts first become detectable in day 6.5 of embryogenesis. B-F cell-surface expression first becomes detectable in hemopoietic organs by day 10‑12 of embryogenesis and somewhat later in nonhemopoietic organs. Flow cytometry analysis of hemopoietic cells throughout embryogenesis revealed B-Fhi and B-F1 cell populations. The percentage of B-F cells in spleen and bone marrow decreased around hatching, which could reflect either cell flows in these organs during this period or the sensitivity of hemopoietic cells to hatching stress.