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Developmental Immunology
Volume 2 (1992), Issue 4, Pages 273-284

A New Marker on Chicken Hematopoietic Cells is Defined by a Monoclonal Antibody Raised Against a V ß Chain of the Human TCR

1lnstitut Jacques Monod, CNRS et Universitd Paris 7, 2 Place Jussieu, Tour 43, Paris Cedex 05 75251, France
2Institut d’Embryologie Cellulaire et Moléulaire, CNRS et Collège de France, 49 avenue de la Belle Gabrielle, Nogent-sur-Marne, 94736, France
3Centre de Recherches Sanofi, Rue du Pr. J. Blayac, Montpellier Cedex 34082, France
4Faculté de Médecine, 27 boulevard Jean Moulin, Marseille Cedex 05 13385, France
5Inserm U277, Institut Pasteur, 25-28 rue du Dr. Roux, Paris Cedex 15 75724, France

Copyright © 1992 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In this paper, we show that a mouse monoclonal antibody, 111-427, specific for the V ß 5.3 chain of the human T-cell receptor (TCR) for antigen, also reacts with chicken hematopoietic cells. Our data indicate that the majority of 111-427 positive cells among peripheral blood leucocytes (PBL) are thrombocytes. This antibody also recognizes two in vitro cell lines, III-C5, an IL-2-dependent T-cell-line and HD11, a macrophage cell line. In addition, erythrocytes and a minor subpopulation of thymus and spleen cells are also stained by the monoclonal antibody (mAb). No specific immunoprecipitation could be detected from 125I radiolabeled cell lysates. By Western blotting techniques, the 111- 427 mAb identifies a single band of apparent molecular weight 91 kD, unaffected by reduction, from III-C5 and HD11 cell lysates. This band is absent in negative cell control lysates. On thrombocytes, the apparent molecular weight of the band is shifted to 87 kD. These results indicate that the mAb does not recognize the chicken T-cell receptor for antigen, but a cell surface marker shared primarily between thrombocytes and erythrocytes. This new chicken cell marker is compared to other cell surface markers in avian or mammalian species that present some analogies in their tissue distribution.