Journal of Immunology Research

Journal of Immunology Research / 1998 / Article

Open Access

Volume 5 |Article ID 062380 | https://doi.org/10.1155/1998/62380

F. Kohen, L. Abel, A. Sharp, Y. Amir-Zaltsman, D. Sömjen, S. Luria, G. Mor, A. Knyszynski, H. Thole, A. Globerson, "Estrogen-Receptor Expression and Function in Thymocytes in Relation to Gender and Age", Journal of Immunology Research, vol. 5, Article ID 062380, 9 pages, 1998. https://doi.org/10.1155/1998/62380

Estrogen-Receptor Expression and Function in Thymocytes in Relation to Gender and Age

Received05 Mar 1997
Revised18 Jun 1997
Accepted24 Jul 1997

Abstract

The expression of estrogen receptor (ER) in thymocytes was studied in young, middle-aged, and old (2, 12, and 24 months, respectively) female and male C57BL/6J mice. Western immunoblots prepared from the thymocytes of females of all age groups showed the presence of a 67-kD protein band, which has been associated with the apparent MW of denatured ER. Flow cytometry analysis o,f cells stained with a monoclonal anti-ER antibody (clone 13H2) disclosed ER expression in both females and males of all age groups. In vivo treatment with estradiol (E2) led to an increase in the specific activity of thymic creatine kinase (CK) in the female mice, whereas the male thymocytes responded with an increase in CK activity only on treatment with dihydrotestosterone (DHT). The data show no differences in ER expression between male and females, but the receptor appears not to be functional in males. Interestingly, when estradiol was applied to co-cultures of lymphoid-depleted fetal thymus (FT) explants and bone-marrow cells, or thymocytes, from young and old females, it resulted in increased cellularity of cultures containing cells of the young, and not those of the old. The proportion of CD4/CD8 phenotypes of the developing cells in these cultures was not affected by E2 treatment. These observations provide a new insight into ER expression and function in T-cell development in relation to gender and age.

Copyright © 1998 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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