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Developmental Immunology
Volume 9, Issue 2, Pages 103-111
http://dx.doi.org/10.1080/1044667021000096455

Autoimmune Cholangitis in the SJL/J Mouse is Antigen Non-specific

1Division of Rheumatology, Allergy and Clinical Immunology, University of California School of Medicine, Davis, CA 95616, USA
2Department of Human Pathology, Kanazawa University Graduate School of Medicine, Kanazawa, Japan
3Division of Liver Diseases, Mount Sinai School of Medicine, New York, NY 10029, USA
4Department of Pathology, Mount Sinai School of Medicine, New York, NY 10029, USA
5Department of Microbiology, Monash University, Clayton, Vic. 3168, Australia

Copyright © 2002 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Primary biliary cirrhosis (PBC) is an autoimmune disease characterized by intrahepatic bile duct destruction and the production of anti-mitochondrial antibodies (AMA). The absence of an animal model has been a striking impedance in defining the molecular basis of disease. Previous work has suggested that SJL/J mice immunize with the pyruvate dehydrogenase complex (PDC-E2), the major mitochondrial autoantigen of PBC, leads to the development of lymphoid cell infiltration in portal tracts and a model system coined autoimmune cholangitis. We hypothesized that this pathology would be augmented if immunization occurred in the presence of IFN-γ injections. Accordingly, SJL/J mice were immunized with PDC-E2 and, for purpose of control, α-casein. Subgroups of mice were also treated with exogenous IFN-γ. As expected, mice immunized with PDC-E2, with or without IFN-γ, developed high titer AMAs. In contrast, mice immunized with α-casein, develop antinuclear antibodies. More importantly, the livers from mice immunized with PDC-E2 and/or those immunized with α-casein all displayed lymphoid cell infiltration to the portal tracts, irrespective of bile duct size. Indeed, there was no significant difference between the experimental and the control groups by histologic analysis. Thus, autoimmune cholangitis in these mice is antigen non-specific.