Comparative Study of Regulatory T Cell Function of Human T Cells from Thymocytes, Cord Blood, and Adult Peripheral Blood
Figure 2
Lack of suppressive activity and absence
of an anergic state by CB CD25+CD4+ T cells.
CD4+CD25+ and CD4+CD25− T cells
were purified by a magnetic microbead system. (a) Cells were lysed and immunoblotted with
anti-FOXP3 Ab (top panel) or anti-β-actin Ab (bottom panel). (b) Cells were stimulated with anti-CD3/CD28-mAb-coated beads under
the indicated conditions. Cell proliferation was assessed by 3H-thymidine
uptake on day 4. Data are representative
of 5 different donors.
