Comparative Study of Regulatory T Cell Function of Human T Cells from Thymocytes, Cord Blood, and Adult Peripheral Blood
Figure 3
Suppressive effect of CD4+CD25+ T cells on cytokine
production by CD4+CD25− responder
cells. CD4+CD25+ T cells
and CD4+CD25− responder cells were prepared by a magnetic
microbead system. Cells were stimulated
with anti-CD3/CD28-mAb-coated beads in the presence of antigen-presenting
cells. The supernatants were removed
after 48 hours of incubation with either CD4+CD25+ T
cells (black bars), CD4+CD25− responder cells (white
bars), and cocultured cells
(gray bars). TGF-β level in supernatants was measured
using ELISA, and levels of other cytokines were measured using a cytometric
bead array kit. Data are mean
of 3 independent experiments.