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Clinical and Developmental Immunology
Volume 2008, Article ID 327346, 8 pages
http://dx.doi.org/10.1155/2008/327346
Clinical Study

Chronic Brucellosis Patients Retain Low Frequency of CD4+ T-Lymphocytes Expressing CD25 and CD28 after Escherichia coli LPS Stimulation of PHA-Cultured PBMCs

Clinical Immunology Unit, 2nd Department of Internal Medicine, Hippokration General Hospital, Aristotle University of Thessaloniki, Konstantinoupoleos Street 49, 546 42 Thessaloniki, Greece

Received 29 July 2008; Revised 20 October 2008; Accepted 19 November 2008

Academic Editor: Bernhard Fleischer

Copyright © 2008 Panagiotis Skendros et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. G. Pappas, N. Akritidis, M. Bosilkovski, and E. Tsianos, “Brucellosis,” The New England Journal of Medicine, vol. 352, no. 22, pp. 2325–2336, 2005. View at Publisher · View at Google Scholar · View at PubMed
  2. G. Pappas, P. Papadimitriou, N. Akritidis, L. Christou, and E. V. Tsianos, “The new global map of human brucellosis,” The Lancet Infectious Diseases, vol. 6, no. 2, pp. 91–99, 2006. View at Publisher · View at Google Scholar · View at PubMed
  3. P. Yagupsky and E. J. Baron, “Laboratory exposures to brucellae and implications for bioterrorism,” Emerging Infectious Diseases, vol. 11, no. 8, pp. 1180–1185, 2005. View at Google Scholar
  4. Z. A. Memish and H. H. Balkhy, “Brucellosis and international travel,” Journal of Travel Medicine, vol. 11, no. 1, pp. 49–55, 2004. View at Google Scholar
  5. S. Al Dahouk, H. Neubauer, A. Hensel et al., “Changing epidemiology of human brucellosis, Germany, 1962–2005,” Emerging Infectious Diseases, vol. 13, no. 12, pp. 1895–1900, 2007. View at Google Scholar
  6. E. Gotuzzo and C. Carrillo, “Brucella,” in Infectious Diseases, S. L. Gorbach, J. G. Bartlett, and N. R. Blacklow, Eds., pp. 1837–1845, W.B. Saunders, Philadelphia, Pa, USA, 2nd edition, 1998. View at Google Scholar
  7. E. F. Berbari and W. R. Wilson, “Brucella, Francisella, Pasteurella, Yersinia and HACEK,” in Current Diagnosis & Treatment in Infectious Diseases, W. R. Wilson and M. A. Sande, Eds., pp. 630–631, McGraw-Hill, New York, NY, USA, 2001. View at Google Scholar
  8. J. de Bagues Maria-Pilar, S. Dudal, J. Dornand, and A. Gross, “Cellular bioterrorism: how Brucella corrupts macrophage physiology to promote invasion and proliferation,” Clinical Immunology, vol. 114, no. 3, pp. 227–238, 2005. View at Publisher · View at Google Scholar · View at PubMed
  9. U. E. Schaible, H. L. Collins, and S. H. E. Kaufmann, “Confrontation between intracellular bacteria and the immune system,” Advances in Immunology, vol. 71, pp. 267–377, 1999. View at Publisher · View at Google Scholar
  10. B. Golding, D. E. Scott, O. Scharf et al., “Immunity and protection against Brucella abortus,” Microbes and Infection, vol. 3, no. 1, pp. 43–48, 2001. View at Publisher · View at Google Scholar
  11. S. Yingst and D. L. Hoover, “T cell immunity to brucellosis,” Critical Reviews in Microbiology, vol. 29, no. 4, pp. 313–331, 2003. View at Publisher · View at Google Scholar
  12. E. Billard, C. Cazevieille, J. Dornand, and A. Gross, “High susceptibility of human dendritic cells to invasion by the intracellular pathogens Brucella suis, B. abortus, and B. melitensis,” Infection and Immunity, vol. 73, no. 12, pp. 8418–8424, 2005. View at Publisher · View at Google Scholar · View at PubMed
  13. S. P. Salcedo, M. I. Marchesini, H. Lelouard et al., “Brucella control of dendritic cell maturation is dependent on the TIR-containing protein Btp1,” PLoS Pathogens, vol. 4, no. 2, p. e21, 2008. View at Publisher · View at Google Scholar · View at PubMed
  14. M. B. Zaitseva, H. Golding, M. Betts et al., “Human peripheral blood CD4+ and CD8+ T cells express Th1-like cytokine mRNA and proteins following in vitro stimulation with heat-inactivated Brucella abortus,” Infection and Immunity, vol. 63, no. 7, pp. 2720–2728, 1995. View at Google Scholar
  15. C. L. Baldwin and R. Goenka, “Host immune responses to the intracellular bacteria Brucella: does the bacteria instruct the host to facilitate chronic infection?,” Critical Reviews in Immunology, vol. 26, no. 5, pp. 407–442, 2006. View at Google Scholar
  16. E. Billard, J. Dornand, and A. Gross, “Brucella suis prevents human dendritic cell maturation and antigen presentation through regulation of tumor necrosis factor alpha secretion,” Infection and Immunity, vol. 75, no. 10, pp. 4980–4989, 2007. View at Publisher · View at Google Scholar · View at PubMed
  17. G. H. Giambartolomei, M. V. Delpino, M. E. Cahanovich et al., “Diminished production of T helper 1 cytokines correlates with T cell unresponsiveness to Brucella cytoplasmic proteins in chronic human brucellosis,” The Journal of Infectious Diseases, vol. 186, no. 2, pp. 252–259, 2002. View at Publisher · View at Google Scholar · View at PubMed
  18. E. Zacharioudaki, P. Boura, I. Kountouras, P. Skendros, and N. Lefkos, “Cytokine regulation and establishment of chronicity in brucellosis,” Immunology Letters, vol. 87, p. 51, 2003, abstract EFIS. View at Google Scholar
  19. A. Rafiei, S. K. Ardestani, A. Kariminia, A. Keyhani, M. Mohraz, and A. Amirkhani, “Dominant Th1 cytokine production in early onset of human brucellosis followed by switching towards Th2 along prolongation of disease,” Journal of Infection, vol. 53, no. 5, pp. 315–324, 2006. View at Publisher · View at Google Scholar · View at PubMed
  20. S. Kinikli, N. Turkcapar, M. B. Kucukay, G. Keskin, and G. Kinikli, “In vitro nonspecific mitogenic response of T-cell subsets in acute and chronic brucellosis,” Diagnostic Microbiology and Infectious Disease, vol. 52, no. 3, pp. 229–233, 2005. View at Publisher · View at Google Scholar · View at PubMed
  21. P. Skendros, P. Boura, D. Chrisagis, and M. Raptopoulou-Gigi, “Diminished percentage of CD4+ T-lymphocytes expressing interleukine-2 receptor alpha in chronic brucellosis,” Journal of Infection, vol. 54, no. 2, pp. 192–197, 2007. View at Publisher · View at Google Scholar · View at PubMed
  22. D. J. Lenschow, T. L. Walunas, and J. A. Bluestone, “CD28/B7 system of T cell costimulation,” Annual Review of Immunology, vol. 14, pp. 233–258, 1996. View at Publisher · View at Google Scholar · View at PubMed
  23. P. Skendros, P. Boura, F. Kamaria, and M. Raptopoulou-Gigi, “CD80/CD28 co-stimulation in human brucellosis,” Clinical and Experimental Immunology, vol. 146, no. 3, pp. 400–408, 2006. View at Publisher · View at Google Scholar · View at PubMed
  24. N. Lapaque, I. Moriyon, E. Moreno, and J.-P. Gorvel, “Brucella lipopolysaccharide acts as a virulence factor,” Current Opinion in Microbiology, vol. 8, no. 1, pp. 60–66, 2005. View at Publisher · View at Google Scholar · View at PubMed
  25. P. G. Cardoso, G. C. Macedo, V. Azevedo, and S. C. Oliveira, “Brucella spp noncanonical LPS: structure, biosynthesis, and interaction with host immune system,” Microbial Cell Factories, vol. 5, article 13, pp. 1–11, 2006. View at Publisher · View at Google Scholar · View at PubMed
  26. E. Billard, J. Dornand, and A. Gross, “Interaction of Brucella suis and Brucella abortus rough strains with human dendritic cells,” Infection and Immunity, vol. 75, no. 12, pp. 5916–5923, 2007. View at Publisher · View at Google Scholar · View at PubMed
  27. J. Goldstein, T. Hoffman, C. Frasch et al., “Lipopolysaccharide (LPS) from Brucella abortus is less toxic than that from Escherichia coli, suggesting the possible use of B. abortus or LPS from B. abortus as a carrier in vaccines,” Infection and Immunity, vol. 60, no. 4, pp. 1385–1389, 1992. View at Google Scholar
  28. M. Guha and N. Mackman, “LPS induction of gene expression in human monocytes,” Cellular Signalling, vol. 13, no. 2, pp. 85–94, 2001. View at Publisher · View at Google Scholar
  29. T. Mattern, A. Thanhäuser, N. Reiling et al., “Endotoxin and lipid A stimulate proliferation of human T cells in the presence of autologous monocytes,” Journal of Immunology, vol. 153, no. 7, pp. 2996–3004, 1994. View at Google Scholar
  30. A. J. Ulmer, H.-D. Flad, Th. Rietschel, and T. Mattern, “Induction of proliferation and cytokine production in human T lymphocytes by lipopolysaccharide (LPS),” Toxicology, vol. 152, no. 1–3, pp. 37–45, 2000. View at Publisher · View at Google Scholar
  31. E. J. Chien, C.-C. Chen, C.-H. Chien, T.-P. Yeh, and L.-M. Lu, “Activation and up-regulation of phospholipase D expression by lipopolysaccharide in human peripheral T cells,” Chinese Journal of Physiology, vol. 47, no. 4, pp. 203–209, 2004. View at Google Scholar
  32. A. K. De, C. L. Miller-Graziano, S. E. Calvano et al., “Selective activation of peripheral blood T cell subsets by endotoxin infusion in healthy human subjects corresponds to differential chemokine activation,” Journal of Immunology, vol. 175, no. 9, pp. 6155–6162, 2005. View at Google Scholar
  33. M. J. Corbel, “Brucellosis: an overview,” Emerging Infectious Diseases, vol. 3, no. 2, pp. 213–221, 1997. View at Google Scholar
  34. M. Zaitseva, H. Golding, J. Manischewitz, D. Webb, and B. Golding, “Brucella abortus as a potential vaccine candidate: induction of interleukin-12 secretion and enhanced B7.1 and B7.2 and intercellular adhesion molecule 1 surface expression in elutriated human monocytes stimulated by heat-inactivated B. abortus,” Infection and Immunity, vol. 64, no. 8, pp. 3109–3117, 1996. View at Google Scholar
  35. S. Hodge, G. Hodge, R. Flower, and P. Han, “Surface and intracellular interleukin-2 receptor expression on various resting and activated populations involved in cell-mediated immunity in human peripheral blood,” Scandinavian Journal of Immunology, vol. 51, no. 1, pp. 67–72, 2000. View at Publisher · View at Google Scholar
  36. A. Hutchings, W. M. Purcell, and M. R. Benfield, “Peripheral blood antigen-presenting cells from African-Americans exhibit increased CD80 and CD86 expression,” Clinical and Experimental Immunology, vol. 118, no. 2, pp. 247–252, 1999. View at Publisher · View at Google Scholar
  37. M. Refik, N. Mehmet, R. Durmaz, and Y. Ersoy, “Cytokine profile and nitric oxide levels in sera from patients with brucellosis,” Brazilian Journal of Medical and Biological Research, vol. 37, no. 11, pp. 1659–1663, 2004. View at Publisher · View at Google Scholar
  38. A. C. Makis, E. Galanakis, E. C. Hatzimichael, Z. L. Papadopoulou, A. Siamopoulou, and K. L. Bourantas, “Serum levels of soluble interleukin-2 receptor alpha (sIL-2Rα) as a predictor of outcome in brucellosis,” Journal of Infection, vol. 51, no. 3, pp. 206–210, 2005. View at Publisher · View at Google Scholar · View at PubMed
  39. K. Ahmed, K. A. Al-Matrouk, G. Martinez, K. Oishi, V. O. Rotimi, and T. Nagatake, “Increased serum levels of interferon-γ and interleukin-12 during human brucellosis,” American Journal of Tropical Medicine and Hygiene, vol. 61, no. 3, pp. 425–427, 1999. View at Google Scholar
  40. K. Demirdag, M. Ozden, A. Kalkan, A. Godekmerdan, and S. S. Kilic, “Serum cytokine levels in patients with acute brucellosis and their relation to the traditional inflammatory markers,” FEMS Immunology and Medical Microbiology, vol. 39, no. 2, pp. 149–153, 2003. View at Publisher · View at Google Scholar
  41. T. Muchamuel, S. Menon, P. Pisacane, M. C. Howard, and D. A. Cockayne, “IL-13 protects mice from lipopolysaccharide-induced lethal endotoxemia: correlation with down-modulation of TNF-α, IFN-γ, and IL-12 production,” Journal of Immunology, vol. 158, no. 6, pp. 2898–2903, 1997. View at Google Scholar
  42. T. Mattern, H.-D. Flad, L. Brade, E. T. Rietschel, and A. J. Ulmer, “Stimulation of human T lymphocytes by LPS is MHC unrestricted, but strongly dependent on B7 interactions,” Journal of Immunology, vol. 160, no. 7, pp. 3412–3418, 1998. View at Google Scholar
  43. P. Boura, G. Tsapas, J. Kountouras, and E. Zaharioudaki, “Interferon-A 2a administration in chronic anergic brucellosis,” Hepato-Gastroenterology, vol. 42, no. 6, pp. 919–922, 1995. View at Google Scholar
  44. P. Boura, P. Skendros, J. Kountouras, E. Zacharioudaki, and T. Tsapas, “Effect of bacterial extracts on the immunologic profile in chronic relapsing Brucellosis patients,” International Journal of Immunopathology and Pharmacology, vol. 12, no. 2, pp. 103–111, 1999. View at Google Scholar
  45. M. A. Dobrovolskaia and S. N. Vogel, “Toll receptors, CD14, and macrophage activation and deactivation by LPS,” Microbes and Infection, vol. 4, no. 9, pp. 903–914, 2002. View at Publisher · View at Google Scholar
  46. W. Heagy, C. Hansen, K. Nieman, J. L. Rodriguez, and M. A. West, “Impaired mitogen-activated protein kinase activation and altered cytokine secretion in endotoxin-tolerant human monocytes,” The Journal of Trauma-Injury Infection & Critical Care, vol. 49, no. 5, pp. 806–814, 2000. View at Publisher · View at Google Scholar
  47. F. N. Lauw, T. ten Hove, P. E. P. Dekkers, E. de Jonge, S. J. H. van Deventer, and T. van der Poll, “Reduced Th1, but not Th2, cytokine production by lymphocytes after in vivo exposure of healthy subjects to endotoxin,” Infection and Immunity, vol. 68, no. 3, pp. 1014–1018, 2000. View at Publisher · View at Google Scholar
  48. T. K. Varma, T. E. Toliver-Kinsky, C. Y. Lin, A. P. Koutrouvelis, J. E. Nichols, and E. R. Sherwood, “Cellular mechanisms that cause suppressed gamma interferon secretion in endotoxin-tolerant mice,” Infection and Immunity, vol. 69, no. 9, pp. 5249–5263, 2001. View at Publisher · View at Google Scholar
  49. H. Reiser and M. J. Stadecker, “Costimulatory B7 molecules in the pathogenesis of infectious and autoimmune diseases,” The New England Journal of Medicine, vol. 335, no. 18, pp. 1369–1377, 1996. View at Publisher · View at Google Scholar
  50. N. L. Harris and F. Ronchese, “The role of B7 costimulation in T-cell immunity,” Immunology and Cell Biology, vol. 77, no. 4, pp. 304–311, 1999. View at Publisher · View at Google Scholar
  51. G. Martínez de Tejada, J. Pizarro-Cerdá, E. Moreno, and I. Moriyón, “The outer membranes of Brucella spp. are resistant to bactericidal cationic peptides,” Infection and Immunity, vol. 63, no. 8, pp. 3054–3061, 1995. View at Google Scholar
  52. C. M. Fernandez-Prada, E. B. Zelazowska, M. Nikolich et al., “Interactions between Brucella melitensis and human phagocytes: bacterial surface O-polysaccharide inhibits phagocytosis, bacterial killing, and subsequent host cell apoptosis,” Infection and Immunity, vol. 71, no. 4, pp. 2110–2119, 2003. View at Publisher · View at Google Scholar
  53. M. G. Rittig, A. Kaufmann, A. Robins et al., “Smooth and rough lipopolysaccharide phenotypes of Brucella induce different intracellular trafficking and cytokine/chemokine release in human monocytes,” Journal of Leukocyte Biology, vol. 74, no. 6, pp. 1045–1055, 2003. View at Publisher · View at Google Scholar · View at PubMed
  54. C. M. Fernandez-Prada, M. Nikolich, R. Vemulapalli et al., “Deletion of wboA enhances activation of the lectin pathway of complement in Brucella abortus and Brucella melitensis,” Infection and Immunity, vol. 69, no. 7, pp. 4407–4416, 2001. View at Publisher · View at Google Scholar · View at PubMed
  55. A. Kariminia, G. Kavoossy, S. Khatami, E. Zowghi, and S. K. Ardestani, “Study of interleukin-10 and interleukin-12 productions in response to lipopolysaccharides extracted from two different Brucella strains,” Comparative Immunology, Microbiology and Infectious Diseases, vol. 25, no. 2, pp. 85–93, 2002. View at Publisher · View at Google Scholar
  56. P. Barrionuevo, J. Cassataro, M. V. Delpino et al., “Brucella abortus inhibits major histocompatibility complex class II expression and antigen processing through interleukin-6 secretion via Toll-like receptor 2,” Infection and Immunity, vol. 76, no. 1, pp. 250–262, 2008. View at Publisher · View at Google Scholar · View at PubMed