Lack of Disease Specificity Limits the Usefulness of In Vitro Costimulation in HIV- and HCV-Infected Patients
Figure 3
In
vitro costimulation causes false-positive T cell responses to HCV and HIV
antigens in healthy individuals. PBMCs of 6 healthy individuals were
tested in IFN-γ ELISPOT assays for their recall responses to NS3 protein,
Pepset 4 peptide pool, and HIV peptide pools 1, 2, and 3 in the absence (a) or
presence of IL-7/IL-15 (b), IFN-α (c), anti-ICOS (d), or CD28 (e). For each individual, the mean spot count of
duplicate wells is shown. Horizontal
bars indicate the means of all individual responses to the respective
antigens. P values are given for
means that are significantly different from baseline values in (a). Symbols in (a) also apply to (b)–(e).
