A Possible Link between Infection with Burkholderia Bacteria and Systemic Lupus Erythematosus Based on Epitope Mimicry

Figure 3

Testing 4 affinity purified human IgG anti-dsDNA antibodies in binding to the purified bacterial antigen in ELISA. ELISA plates were coated with the purified bacterial antigen (50 𝜇g/mL). Lanes 1–4 contain purified anti-dsDNA antibodies (1 𝜇g/100 𝜇L) binding to purified bacterial antigen and lane 5 has CF II as control binding to purified bacterial antigen. Anti human IgG Fc fragment specific alkaline phosphatase conjugate was used to detect anti-dsDNA antibody binding. The data were expressed as the mean ± SD.