Methotrexate and Cyclosporine Treatments Modify the Activities of Dipeptidyl Peptidase IV and Prolyl Oligopeptidase in Murine Macrophages

Table 1

Dipeptidyl peptidase IV (DPPIV) and prolyl oligopeptidase (POP) activities in soluble (S) and membrane-bound (M) fractions of resident (RE) and thioglycollate-elicited (TGE) macrophages from vehicle-treated animals (ricine oil s.c.= controls of cyclosporine; saline p.o.= controls of methotrexate; ricine oil s.c. plus saline p.o.= controls of methotrexate plus cyclosporine).

Vehicle	Enzyme	Activity (UP/mg protein)
		S		M
		RE	TGE	RE	TGE
Ricine oil	DPPIV	𝑃<.05	𝜙	𝜙	𝜙
Saline		𝜙	𝜙	𝜙	𝜙
Ricine oil + saline		𝜙	𝜙	𝜙	𝜙
Ricine oil	POP	334±42	359±53^∗	absent
Saline		112±7	112±26	absent
Ricine oil + saline		391±67	174±35^a	absent

UP= picomoles substrate hydrolyzed per minute. Values are means ± SEM from 5 animals (assays made in triplicate). Comparisons among vehicle treatments regarding the same enzyme activity in each fraction and macrophage status (analysis of variance, ANOVA, followed by SNK test): 145±43, 73±12 versus saline; 319±23 versus saline or ricine oil. Comparisons between TGE versus RE related to the same enzyme activity in each fraction and vehicle treatment (unpaired two-sided Student’s t-test): 357±13^∗∗, 605±50^∗∗∗, 355±53^∗∗∗b.