The Protective Effects of Ultraviolet A1 Irradiation on Spontaneous Lupus Erythematosus-Like Skin Lesions in MRL/lpr Mice
Figure 5
Analysis of the production of IL-10 by mast cells in MRL/lpr mice. (a), (b), (c): spontaneous LE-like skin lesion of nonirradiated mice. (d), (e), (f): nonlesional skin of UVA1-irradiated (10 J/c) mice. Mast cells are labeled with avidin-Texas Red in red, while IL-10 is immunostained with anti-IL10 mAb and Alexa Fluor 488-conjugated secondary Ab in green. In the LE-like skin lesions, some double immunopositive IL-10-producing mast cells were seen (yellow in (c), indicated by arrows). In contrast, in the nonlesional skin of UVA1-irradiated (10 J/c) mice, such cells were not detected (f). (a), (d): immunostain for IL-10, (b), (e): immunostain for mast cells, (c), (f): merged images, original magnification, .
