850623.fig.002a
(a)
850623.fig.002b
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850623.fig.002c
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850623.fig.002d
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Figure 2: The putative antigen in heat-stressed HGC-27 cells was detected as EF-2K . (a) Western blotting of HGC-27 cells and heat-stressed HGC-27 cells by the sera of GU and CG. Lane 1; molecular weight marker, lanes 2–4; the proteins from heat-stressed HGC-27 cells reacted by GU sera, lanes 5–7; the proteins from heat-stressed HGC-27 cells reacted by CG sera, lanes 8–10; the proteins from nonstressed HGC-27 cells reacted by GU sera, lanes 11–13; the proteins from nonstressed HGC-27 cells reacted by CG sera. 90 kDa protein from heat-stressed HGC-27 cells reacted by GU sera specifically. (b) The expression and purification of EF-2K recombinant protein. About 100 kDa protein was purified. (c) Antibody to EF-2K recombinant protein in patients with GU and CG. The titer of anti-EF-2K recombinant protein antibodies in GU was significantly higher than that in CG (* 𝑃 < . 0 5 ). (d) Quantification of EF-2K expressions in HGC-27 infected by H. pylori. The EF-2K expressions in HGC-27 were enhanced by H. pylori infection as by heat stress.