The SSX Family of Cancer-Testis Antigens as Target Proteins for Tumor Therapy

Table 3

SSX epitopes: sequences, haplotype restrictions, and recognition. The known SSX immunogenic class I and class II MHC peptides or epitopes are shown. Amino acid sequence, haplotype restriction, tumor presentation, family member recognition, and references are outlined for each peptide/epitope. Symbol designations include + = SSX4 sequence, n.d. = not demonstrated. ^† = p41–49 epitope shown to be recognized on Me 275, SK-MEL-37, T343B, and T567A melanoma cells and SW 872 liposarcoma cells; p103–111 shown to be recognized on SK-MEL-37 melanoma cells and LNCaP prostate cancer cells; and p45–59 shown to be recognized on Me 275 cells. * = Recognized on antigen-loaded dendritic cells but not endogenously presented by tumor cells.

Class	Name	Native peptide sequence	Haplotype	Naturally presented by SSX+ tumor cells	Known family member recognition	Reference
I	p41–49	KASEKIFYV	HLA-A*0201	Y^†	SSX2	[104–106]
	p57–65	AMTKLGFKA	HLA-A*0201	n.d.	SSX1–9	[107]
	p103–111	RLQGISPKI	HLA-A*0201	Y^†	SSX2	[108, 109]
II	p19–34	EKIQKAFDDIAKYFS	HLA-DPB1*0101	n.d.*	SSX2	[110]
	p37–51	WEKMKASEKIFYVYM	HLA-DR3*0301	n.d.*	SSX2, SSX4, SSX5	[111]
	p45–59	KIFYVYMKRKYEAMT	HLA-DRB1*1101, *0701, *1101, *1302, and B3*0301	Y^†	SSX2	[112, 113]
	p21–40	RSKAFDDIATYFSKKEWKKM	HLA-DRB1*1501	n.d.*	SSX1	[114]
	p31–50	YFSKKEWEKMKSSEKIVYVY+	HLA-DRB*0301 and *1101 (SSX4)	n.d.*	SSX1, SSX4	[114, 115]
	p41–60	KSSEKIVYVYMKLNYEVMTK+	HLA-DRB1*1501 or DRB5*0101 (SSX4) and HLA-DR1*1601 (SSX1)	n.d.*	SSX1, SSX4	[114, 115]
	p51–70	MKLNYEVMTKLGFKVTLPPFM+	HLA-DRB1*0701 (SSX4)	n.d.*	SSX1, SSX4	[103, 114, 115]
	p61–80	LGFKVTLPPFMRSKRAADFH	HLA-DRB1*1101	n.d.*	SSX4	[115]
	p101–120	FGSLQRIFPKIMPKKPAEEE+	HLA-DRB1*1101 (SSX4)	n.d.*	SSX1, SSX4	[114, 115]
	p141–160	PPGKANISEKINKRSGPKRG	HLA-DR1*1601	n.d.*	SSX1	[114]
	p151–170	INKTSGPKRGKHAWTHRLRE	HLA-DPB1*1001	n.d.*	SSX4	[115]
	p161–180	KHAWTHRLRERKQLVVYEEI	HLA-DRB1*08 and HLA-DRB3*0202	n.d.	SSX4	[103]