CpG Oligodeoxynucleotides Enhance the Efficacy of Adoptive Cell Transfer Using Tumor Infiltrating Lymphocytes by Modifying the Th1 Polarization and Local Infiltration of Th17 Cells
Figure 5
CpG-ODNs enhanced the infiltration of Th17 cells in tumor mass. TILs were collected from lung cancer patients () and treated with CpG-ODNs or control CpG-ODNs as described in Materials and Methods. autologous TILs were transferred into the tumor bearing Balb/c nude mice with 100 ug CpG-ODNs or control CpG-ODNs through tail vein. Ten days later, the percentages of IL-17 positive cells in CD4+ T cells isolated from tumor mass were detected by flow cytometry. Representative data from 12 individual patients with lung cancer was shown (a), and the mean frequency of Th17 cells also was calculated, respectively (b). .
