Tumor Antigen-Dependent and Tumor Antigen-Independent Activation of Antitumor Activity in T Cells by a Bispecific Antibody-Modified Tumor Vaccine
Figure 4
In vivo antitumor activities of human PBMC preactivated in vitro by the NDV-based tumor vaccine of the second generation. (A) Experimental protocol. MCF-7 tumor cells were injected subcutaneously into NOD/SCID mice ( per group). The mice were kept until palpable tumors were established (6–8 mm in diameter) (a). Then, at days 1, 4, and 7, the mice were treated by i.p. injection of 107 PBMC which were preactivated ex vivo for 3 days by the NDV-based tumor vaccine of the first or second generation obtained using the MCF-7 cell line (b). Tumor growth was then monitored over time (c). (B) Tumor diameter and representative immunohistochemistry images of tumor tissue sections. Tumor-bearing mice were treated with PBMC activated with MCF7-NDV (left) or with PBMC preactivated with MCF-7-NDV loaded with bsHN-CD3 and bsHN-CD28 (right) and were analyzed over time for tumor growth, and data at day 93 is represented (top). At this time point, some mice were sacrificed, and the tumor sections were stained with mAbs against the human CD8 antigen and analyzed by fluorescence microscopy (bottom). Scale bar, 100 μm (adapted from [42]).
