Clinicopathologic Significance of HIF-1, CXCR4, and VEGF Expression in Colon Cancer
Figure 1
Expression of HIF-1, CXCR4, and VEGF in colon primary tumor samples. Sections were subjected to routine deparaffinization and rehydration. Antigen retrieval was achieved by microwaving in citrate buffer for 10 min. The endogenous peroxidase activity was inhibited by incubation with 3% hydrogen peroxide. The specimens were reacted overnight with anti-HIF-1 antibodies, anti-CXCR4 antibodies, and anti-VEGF antibodies, then were incubated with rat anti-mouse-IgG2b-horseradish peroxidase. The sections were then counterstained with hematoxylin and mounted (see Section 2). HIF-1 staining was observed in the cytoplasm and in the nucleus of the tumor cells, CXCR4, and VEGF staining was observed in the cytoplasm of tumor cells. Positive staining was observed as a brown color. Weakly positive immunohistochemical staining of three molecules in (a) (HIF-1), (c) (CXCR4), (e) (VEGF), respectively (×100). Strongly positive immunohistochemical staining of three molecules in (b) (HIF-1), (d) (CXCR4), (f) (VEGF), respectively, (×100).
