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Clinical and Developmental Immunology
Volume 2011, Article ID 143517, 7 pages
http://dx.doi.org/10.1155/2011/143517
Research Article

Serum Positive for the Autoantibody against the -Adrenoceptor from Chinese Patients with Congestive Heart Failure Decreases in Mouse Cardiac Myocytes

1Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Jinan 250012, China
2Department of Cardiology, QiLu Hospital, Shandong University, Jinan 250012, China
3Department of Cardiology, Shandong Provincial Hospital, Shandong University, Jinan 250021, China

Received 5 November 2010; Accepted 7 April 2011

Academic Editor: Stuart Mannering

Copyright © 2011 Yuan-yuan Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Autoantibodies targeting the β1-adrenergic receptor (AAB-β1) display agonist-like effects, which may have a pathogenic role in the progression of heart failure. Here, we used the electrophysiological recordings to explore the effects of AAB-β1-positive serum from Chinese patients with heart failure on the activity of the peak transient outward potassium current ( ) and the end 50 ms steady-state potassium current ( ) in mouse cardiac myocytes. We found that the AAB-β1-positive serum had no effect on the activity of , but it produced a decrease in the currents of . A low concentration of positive serum (1/100) had a small inhibitory effect on . However, positive serum at 1 : 10, 1 : 20, and 1 : 50 significantly decreased . The concentration-dependence analysis showed that the EC50 of AAB-β1-positive serum was 1/60.24 and its nH was 2.86. It indicated that the AAB-β1 could inhibit in mouse cardiomyocyte in a concentration-dependent manner.