A Functional Polymorphism in B and T Lymphocyte Attenuator Is Associated with Susceptibility to Rheumatoid Arthritis
Figure 2
Loss of inhibitory function of 590C BTLA on ConA- and anti-CD3 Ab-induced IL-2 production in Jurkat T cells. (a) FACS analysis of BTLA expression in stable transfectants of Jurkat T cells with the retrovirus of 590A BTLA-IRES-GFP (solid line), 590C BTLA-IRES-GFP (dotted line), or GFP-RV (a negative control; shaded histogram). Both of transfectants express BTLA at similar levels. (b) ConA-induced IL-2 production from Jurkat T cells expressing 590A BTLA or 590C BTLA. Jurkat T cells expressing 590A BTLA or 590C BTLA (5 × 105 cells) were cocultured with 2 × 105 of HVEM-expressing CHO cells in the presence of various concentrations of concanavalin A (ConA). Twenty-four hours later, IL-2 levels in the supernatants were measured by ELISA. Representative data from three independent experiments are shown. (c) Anti-CD3 Ab-induced IL-2 production from Jurkat T cells expressing 590A BTLA or 590C BTLA. Jurkat T cells expressing 590A BTLA or 590C BTLA (5 × 105 cells) were stimulated with immobilized anti-CD3 Ab (0.2 μg/ml) and various concentrations of immobilized anti-BTLA Ab (MIH26) (0–20 μg/ml) in the presence of anti-CD28 Ab (1 μg/ml). Twenty-four hours later, IL-2 levels in the supernatants were determined by ELISA. Representative data of the percent IL-2 production from three independent experiments are shown.
