Immunopathology of Postprimary Tuberculosis: Increased T-Regulatory Cells and DEC-205-Positive Foamy Macrophages in Cavitary Lesions

Table 1

Conditions for immunohistochemistry staining of immune cell markers and MTB.

Marker	Pretreatment	Dilution	Incubation time^a
CD4	steam for 20 minutes in EDTA, pH 8.0	1 : 25	45
CD8	steam for 20 minutes in EDTA, pH 8.0	1 : 125	30
CD20	5 minutes under pressure in citrate buffer, pH 6.0	1 : 800	20
CD31	5 minutes under pressure in citrate buffer, pH 6.0	1 : 100	30
CD68	5 minutes under pressure in citrate buffer, pH 6.0	1 : 2000	20
DEC-205	steam for 20 minutes in EDTA, pH 8.0	1 : 50	overnight
Foxp3	5 minutes under pressure in citrate buffer, pH 6.0	1 : 100	30
MTB	Proteinase K	1 : 100	60

^aminutes.