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Clinical and Developmental Immunology
Volume 2011, Article ID 351573, 10 pages
Research Article

Human T Cell and Antibody-Mediated Responses to the Mycobacterium tuberculosis Recombinant 85A, 85B, and ESAT-6 Antigens

1Laboratory of Immunology of Infectious Diseases, Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, 31270-901 Belo Horizonte, MG , Brazil
2Department of Parasitology, Microbiology and Immunology, Biological Sciences Institute, Federal University of Juiz de Fora, 36036-900 Juiz de Fora, MG, Brazil
3Oswaldo Cruz Health Center, Belo Horizonte-Minas Gerais, 30180-080 Belo Horizonte, MG, Brazil
4Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, 88040-900 Florianopolis, MG, Brazil

Received 14 September 2010; Revised 1 November 2010; Accepted 5 November 2010

Academic Editor: James Triccas

Copyright © 2011 Gilson C. Macedo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Tuberculosis remains a major health problem throughout the world causing large number of deaths. Effective disease control and eradication programs require the identification of major antigens recognized by the protective responses against M. tuberculosis. In this study, we have investigated humoral and cellular immune responses to M. tuberculosis-specific Ag85A, Ag85B, and ESAT-6 antigens in Brazilian patients with pulmonary (P, ) or extrapulmonary (EP, ) tuberculosis, patients undergoing chemotherapy (PT, ), and noninfected healthy individuals (NI, ). Compared to NI, we observed increased levels of IgG1 responses to Ag85B and ESAT-6 in P and PT groups. Regarding cellular immunity, Ag85A and ESAT-6 were able to discriminate P, PT, and EP patients from healthy individuals by IFN-γ production and P and PT groups from EP individuals by production of TNF-α. In summary, these findings demonstrate the ability of Ag85A, Ag85B, and ESAT-6 to differentiate TB patients from controls by IgG1, IFN-γ and TNF-α production.