Human T Cell and Antibody-Mediated Responses to the Mycobacterium tuberculosis Recombinant 85A, 85B, and ESAT-6 Antigens
Figure 3
Lymphocyte proliferation in response to recombinant Ag85A, Ag85B and ESAT-6. Freshly isolated PBMC from non-infected individuals (NI), pulmonary TB patients (P), pulmonary TB patients in treatment (PT) and extra-pulmonary TB (EP) were cultured in the presence of Ag85A (25 μg/ml), 85B (25 μg/ml), PPD (25 μg/ml) or ESAT-6 (50 μg/ml) for 5 days and incorporation of [3H] thymidine was measured. Results are expressed as stimulation index (SI) mean of triplicate cultures. Horizontal bars indicate mean values. Differences between responses from TB patient groups and control groups were analyzed with nonparametric Kruskall-Wallis test. (*) Statistically significant differences in relation to NI group and (δ) in relation to EP group ().
