Lymph Node Transplantation and Its Immunological Significance in Animal Models

Figure 3

The number of immunoglobulin A (IgA)⁺ cells in the lamina propria is decreased after transplantion of pLN into the mesentery. The gut of mLNtx and pLNtx transplanted animals was analyzed by gating on IgA⁺ cells by flow cytometry. Dot plots of the IgA⁺ cells of mLNtx and pLNtx are shown. Furthermore, immunofluorescence staining of the lamina propria of the gut in mLN transplanted and pLN transplanted rats was carried out with antibodies against IgA (green). Dapi was used to visualize all cells. IgA⁺ cells were seen in both groups, but to a lesser extent in pLNtx animals.

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