Phenotype and Function of CD25-Expressing B Lymphocytes Isolated from Human Umbilical Cord Blood
Figure 1
Expression and functional evaluation of the IL-2R on cord blood and adult CD20+ B cells. From freshly isolated cord blood (N = 10) or adult blood, mononuclear cells (N = 10) were isolated by density gradient centrifugation. Cells were stained for 3-colour flow cytometry analysis using a combination of CD20, CD25, CD122, or CD132. (a) shows the CD25 expression on CD20+ cells isolated from cord blood or adult blood. In (b), the CD122 expression and in (f) the CD132 expression are analysed on CD25+ and CD25− CD20+ B cells isolated from cord blood or adult blood. In (c) the median intensity of CD122 and in (g) the median intensity of CD132 are shown on CD25+ and CD25− CD20+ B cells isolated from cord blood or adult blood. (d) and (h) show a representative histogram of CD122 (d) and CD132 expression on CD25+ and CD25− cord blood B cells. Black line indicates CD25+ and filled histogram represent CD25− cord blood B cells. (e) and (i) show a representative histogram of CD122 (d) and CD132 expression on CD25+ and CD25− adult B cells. Black line indicates CD25+ and filled histogram represent CD25− adult B cells. Finally, in (j), the proliferation of 3H-thymidine labelled sorted CD25+ and CD25−CD20+ B cells isolated from cord blood (N = 6) is shown following 96 h of stimulation with 25U rh-IL-2. Line in boxes represents median. Statistical evaluation was performed using the parametric unpaired t-test when comparing results from cord blood versus results from adult blood, whereas when comparing results within each group, the paired t-test was used. *, **, and ***.
