Newborn Mice Vaccination with BCG.HIVA²²² + MVA.HIVA Enhances HIV-1-Specific Immune Responses: Influence of Age and Immunization Routes

Figure 3

Induction of multifunctional HIV-1-specific CD8⁺ T cells by the BCG.HIVA²²² prime and MVA.HIVA boost regimen. Analysis of bifunctional vaccine-elicited CD8⁺ T cells using the P18I10 peptide stimulation. (a) Example of dot blots and region selection for analysis. Region selected to analyze the splenocytes in the SSC-FSC dot-blot presentation (left), the CD8+ splenocytes selection (middle) and the IFNγ and TNF-α staining of the CD8⁺ splenocytes (right). (b) This panel shows the frequencies of CD8⁺ T cells producing IFN-γ and/or TNF-α. Data are presented as means ± SEM. (c) In vitro analysis of the CTL activity using peptide-pulsed target cells. BCG.HIVA²²² primed mice: striped bars. BCGwt primed mice: white solid bars. ^∗=𝑃<.05; ^∗∗=𝑃<.01, ^∗∗∗=𝑃<.001.

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