Immunogenicity and Protective Efficacy against Murine Tuberculosis of a Prime-Boost Regimen with BCG and a DNA Vaccine Expressing ESAT-6 and Ag85A Fusion Protein

<table>Construction of recombinant plasmids. Genes encoding ESAT-6, Ag85A, the fusion protein of ESAT-6, and Ag85A with a 45 bp linker, were cloned from <i>M. tuberculosis</i> H37Rv genomic DNA, respectively (A). The PCR products of the fusion genes were inserted into the <i>Bam</i>HI and <i>Eco</i>RI sites of pProExHTb or pcDNA3.1, resulting in the recombinant plasmids pPro685A and pcD685A, respectively. The recombinant plasmids were identified by enzyme digestion (B). Lane M: DNA molecular marker; lane A1: PCR product of <i>esat-6</i>; lane A2: PCR product of <i>fbpA</i>; lane A3: PCR product of <i>esat-6-fbpA</i>; lane B1: products of pPro685A digested with enzymes; lane B2: products of pcD685A digested with enzymes.</table>

Journal of Immunology Research

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Figure 1

Figure 1: Immunogenicity and Protective Efficacy against Murine Tuberculosis of a Prime-Boost Regimen with BCG and a DNA Vaccine Expressing ESAT-6 and Ag85A Fusion Protein 

Figure 1 | Immunogenicity and Protective Efficacy against Murine Tuberculosis of a Prime-Boost Regimen with BCG and a DNA Vaccine Expressing ESAT-6 and Ag85A Fusion Protein 