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Journal of Immunology Research
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Journal of Immunology Research
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2011
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Article
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Tab 1
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Research Article
Immunogenicity and Protective Efficacy against Murine Tuberculosis of a Prime-Boost Regimen with BCG and a DNA Vaccine Expressing ESAT-6 and Ag85A Fusion Protein
Table 1
Primers and thermal cycle parameters for cloning of
M. tuberculosis
antigens.
Protein Names
Primer names and sequences (5′-3′)
Cycle parameters
ESAT-6
ESAT6F: GGATCCATGACAGAGCAGCAGTG
ESAT6R: GCTGCCGCCACCGCCGCTTCCGCCACCGCCGCTTCCACCGCCACCTGCGAACATCCCAGTGACGTTGCCTTC
95°C 5 min; 94°C 45 s, 60°C 45 s, 72°C 50 s, 30 cycles; 72°C 10 min
Ag85A
Ag85AF: GGTGGCGGTGGAAGCGGCGGTGGCGGAAGCGGCGGTGGCGGCAGCGCATTTTCCCGGCCGGGCTTG
Ag85AR: GGAATTCTGTTCGGAGCTAGGCGCCCTGGG
95°C 5 min; 94°C 45 s, 60°C 45 s, 72°C 50 s, 30 cycles; 72°C 10 min
r685A
ESAT6F
Ag85AR
95°C 5 min; 94°C 1 min, 60°C 1 min, 72°C 90 s, 30 cycles; 72°C 10 min