NOD Dendritic Cells Stimulated with Lactobacilli Preferentially Produce IL-10 versus IL-12 and Decrease Diabetes Incidence
Figure 2
Incidence of diabetes in NOD mice injected with LTA-treated DCs. BM-DCs from NOD mice were stimulated in vitro overnight with 10 μg/mL of LTA from S. aureus (LTA) or medium alone (none), washed, and one million DCs were injected into six-week-old NOD mice (-6), and blood glucose was monitored weekly (a). Sera from pooled NOD mice untreated or injected with DCs treated with LTA were collected at 28 hrs after injection and tested for the presence of IL-12 and IL-10 by ELISA (b). One million DCs untreated or treated with 1, 10 or 100 μg/mL of LTA were injected into six-week-old NOD mice (–11), and blood glucose was monitored weekly (c). Mice were considered diabetic when their blood glucose levels were >300 dL/mL for 2 consecutive weeks. *indicates a significant difference from the “DC only” or “untreated” group at .
