(a) 33-year-old female ABD patient showed erythema reaction exhibiting more than 10 mm in diameter by self-saliva (salivary prick: S-prick) 48 hours after prick. A tinny spot by sterilized self-saliva (sterilized salivary prick: SS-prick) and control saline (control saline prick: CS-prick) were observed. (b) Histology of the skin reaction by S-prick from 27-year-old male ABD patient. Edema in the upper dermis and many mononuculear cell infiltration around the dilated vessels in the middle dermis were found (HE, 100). The magnified view of the vascular change with infiltrate (200). (c) Immunochemical histology of the skin reaction by S-prick. (avidin-biotin complex peroxydase method: ABC method, 200). Infiltration of CD3+/CD4+ cells is mainly found around vessels and the proportion of infiltrate was CD3+/4+ CD68+ CD8+ cells. No CD20+ and CD56+ cells were found.
