The Role of the IL-12 Cytokine Family in Directing T-Cell Responses in Oral Candidosis
Figure 2
Heat-killed Candida albicans (HKC) stimulated THP-1 cell for Ebi3 and TLR2/4 expression. (a) THP-1 cells were stimulated with HKC /mL and 100 ng/mL LPS for indicated time. The Ebi3 mRNA was detected by RT-PCR. The RNA equal loading was shown by -Actin RT-PCR. (b) THP-1 cells were stimulated with two clinical strains of C. albicans (S1 and S2) for overnight. The Ebi3 expression was presented by band density ratios of Ebi3 over β-Actin. (c) IL-27 cytokineS were measured by a human IL-27 ELISA (R&D system) in a cell culture with pretreated THP-1 cell with indicated increase density of heat killed Candida followed by low dose LPS stimulation. (d) TLR2 and 4 mRNA expression was increased by HKC /mL.
