Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin
Figure 3
Bacterial loads in DC infected with MTB H37Rv, MTB CMT97, BCG Aventis and BCG Japan. DCs obtained from the same donor were exposed to different mycobacteria (MOI, 1). After 3 h, cells were washed then, part of them was checked for intracellular mycobacteria while the rest were resuspended in fresh medium and left in culture. Total bacterial load was assessed over the first 7 days and it is indicated as fold induction compared to the intracellular bacteria after 1 day from infection. The BCG Japan CFU were found significantly different at day 7 as compared to MTB H37Rv (), MTB CMT97 () and BCG Aventis (). Data shown as mean ± SEM from 3 independent experiments performed in triplicates. Statistical significance was assessed by using two-way ANOVA.
