Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin

Table 1

Primer sequences used in the q-rt RT-PCR.

Gene	Sense	Antisense	Annealing temp. (°C)
L34	5′-GGCCCTGCTGCATGTTTCTT-3′	5′-GTCCCGAACCCCTGGTAATAGA-3′	64
EBI3	5′-AGAGCACATCATCAAGCCCGAC-3′	5′-TCCCTGACGCTTGTAAGCGCATC-3′	64
CCR7	5′-AAAAGCGTGCTGGTGGTGGC-3′	5′-ATGATAGGGAGGAACCAGGC-3′	64
TNF-α	5′-AGGCGGTGCTTGTTCCTCA-3′	5′-GTTCGAGAAGATGATCTGACTGCC-3′	60
CCL19	5′-CCAATGATGCTGAAGACTGC-3′	5′-CTGGATGATGCGTTCTACCC-3′	62
CCL20	5′-TGAAGGCTGTGACATCAATGC-3′	5′-TGTTTTGGATTTGCGCACAC-3′	60
IL-12	5′-GCTGCTGAGGAGAGTCGTCCC-3′	5′-CCAGCTGACCTCGACCTGCC-3′	62
IL-10	5′-AGGCGCATGTGAACTCCCT-3′	5′-CACGGTCTTGCTCTTGTTTT-3′	64
IFN-γ	5′-GGCTGTTACTGCCAGGACCCCATATGT-3′	5′-GATGCTCTTGCACCTCGAAACAGCCAT-3′	64
IL-32	5′-GACATGAAGAAGCTGAAGGCC-3′	5′-ATCTGTTGCCTCGGCACCG-3′	62
IL-18	5′-GACAATTGCATCAACTTTGTGG-3′	5′-ATAGAGGCCGATTTCCTTGG-3′	62
c-FLIP	5′-TTCATGGGAGATTCATGCCC-3′	5′-AAGAGGCTGCTGTCCTCCA-3′	60
CD83	5′-AGGTTCCCTACACGGTCTCC-3′	5′-TTGAAGCTGGTAGTGTTTCG-3′	60