Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin
Table 2
(a) Upregulated genes of DCs infected with different MTB and BCG strains. (b) Downregulated genes of DCs infected with different MTB and BCG strains.
(a)
Gene
MTB H37Rv
MTB CMT97
BCG Aventis
BCG Japan
Common name
Classification
Function
ADAM19
+
+
+
+
Metalloproteinase
Migration/Inflammation
BASP1
+
+
+
+
CAP-23, NAP-2
Cell surface protein
Prot-prot interaction
CCL5
+
+
+
+
RANTES
Chemokine ligand
Migration
CCL20
+
+
+
+
MIP-3α
Chemokine ligand
Inflammation
CCL22
+
+
+
+
MDC
Chemokine ligand
Inflammation
CCR7
+
+
+
+
Chemokine receptor
Migration
CD83
+
+
+
+
Cell surface protein
Ag presentation
IL-1β
+
+
+
+
Cytokine
Inflammation
IL-6
+
+
+
+
Cytokine
Inflammation
IL-10
+
+
+
+
Cytokine
Immune regulation
IL-12p40
+
+
+
+
Cytokine
T cell stimulation
LY75
+
+
+
+
DEC-205
Cell surface receptor
Ag presentation
NFKB1
+
+
+
+
Trascriptional factor
Signal transduction
SOD2
+
+
+
+
Mytocondrial protein
Oxidative stress
TAP2
+
+
+
+
Ag transporter 2
Ag presentation
TLR2
+
+
+
+
Toll-like receptor
Pathogen assoc. receptor
TNF-α
+
+
+
+
Cytokine
Inflammation
ADAR
+
+
Adenosine deaminasi
RNA modification
CCL3
+
+
+
MIP-1α
Chemokine ligand
Inflammation
CCL19
+
+
Cell surface receptor
Migration
CD80
+
Cell surface protein
Ag presentation
CD86
+
+
+
Cell surface protein
Ag presentation
CFLAR
+
+
C-Flip
Apoptosis regulator
Apoptosis inhibitor
CRF
+
C1q related factor
EBI3
+
+
Secreted glicoprotein
Immune-regulation
IL-1a
+
+
+
Cytokine
Inflammation
LAMP3
+
+
+
CD63
Lysosomal associated protein
Ag capture
MT2A
+
+
Metallothionein
Oxidative stress
PLAUR
+
+
CD87
Cell surface receptor
Migration
TNFRSF6
+
+
CD95, FAS
Cell surface receptor
Maturation
(b)
Gene
MTB H37Rv
MTB CMT97
BCG Aventis
BCG Japan
Common name
Classification
Function
ARHGDIB
−
−
−
−
RhoGDP dissociation inhibitor 2
Cell motility and adhesion
CD1A
−
−
−
−
MHC I like protein
Ag presentation
CD1B
−
−
−
−
MHC I like protein
Ag presentation
CD1C
−
−
−
−
MHC I like protein
Ag presentation
CD36
−
−
−
−
Cell surface protein
Ag capture
CD68
−
−
−
−
Cell surface protein
Ag capture
CD74
−
−
−
−
Invariant chain
MHC II assoc. protein
Ag loading
CD209
−
−
−
−
DC-SIGN
C-type lectin receptor
T/DCs interaction
CLECSF12
−
−
−
−
DECTIN-1
Cell surface receptor
Pattern recog. receptor
CLECSF6
−
−
−
−
DCIR
C-type lectin receptor
Ag capture
CST3
−
−
−
−
Proteinase inhibitor
CXCL16
−
−
−
−
Chemokine
T cell stimulation
FCER1A
−
−
−
−
Fc receptor
Inflammation
FCER2
−
−
−
−
Fc receptor
Inflammation
GIP3
−
−
−
−
IFN-α inducible protein
HLA-DMA
−
−
−
−
MHC II accessory protein
Ag loading
HLA-DMB
−
−
−
−
MHC II accessory protein
Ag loading
IFI16
−
−
−
−
IFN-γ inducible protein
Cell cycle regulator
IFITM3
−
−
−
−
IFN inducible protein
Immune response
ITGB2
−
−
−
−
Integrin
Migration
LANGERIN
−
−
−
−
Cell surface protein
Ag capture
LIPA
−
−
−
−
Lipase A
Acid lipase
Lipidic metabolism
MX1
−
−
−
−
IFN-α inducible p78
Antiviral response
RNASE6
−
−
−
−
Ribonuclease
Ag presentation
TLR4
−
−
−
−
Toll-like receptor
Pathogen assoc. receptor
CSF1R
−
−
−
CD115
Cell surface receptor
M-CSF receptor
DCP1B
−
−
Decapping enzyme hDcp1b
RNA degradation
DCSTAMP
−
−
Cell trasmemb. protein
GBP3
−
Guanilate binding protein
GIP2
−
−
IFN-α inducible protein
IL-18
−
Cytokine
Inflammation
PFN1
−
TLR6
−
−
Toll-like receptor
Pathogen assoc. receptor
Upregulated (Table 2(a)) and downregulated (Table 2(b)) genes of DCs exposed for 3 h to MTB H37Rv, MTB CMT97, BCG Aventis and BCG Japan as compared to uninfected DCs were assayed by macroarray 1-day culture after infections. For genes to be referred as upregulated or downregulated we considered only those that showed at least a twofold change in the level of RNA as compared to uninfected DCs expression, in at least 2 independent experiments of the 3 performed.