HLA Class II Defects in Burkitt Lymphoma: Bryostatin-1-Induced 17 kDa Protein Restores CD4+ T-Cell Recognition
Figure 1
BLs are deficient in their ability to present Ag in the context of HLA class II. BL (Nalm-6.DR4, Ramos.DR4, and OUS) and B-LCL (6.16.DR4.DM and Frev) were incubated with whole HSA (a and c) or HSA synthetic peptide (b and d). Following incubation, cells were washed and co-cultured with the epitope-specific T-cell hybridoma 17.9. Supernatant from the co-culture was assayed by ELISA to determine IL-2 levels as a measure of T-cell stimulation. All three BL cell lines were deficient in stimulation of IL-2 production for both whole HSA and HSA synthetic peptide, while both B-LCL efficiently presented each Ag to stimulate high levels of IL-2 production.
