Lactobacillus paracasei Reduces Intestinal Inflammation in Adoptive Transfer Mouse Model of Experimental Colitis
Figure 1
ST11 induces phenotypic DC maturation and specific cytokine release pattern. (a) 5 × 105 BM-DCs were cultured for 24 h in the absence (Unstim.) or the presence of ST11 (at bacteria : DC ratio of 100 : 1). As positive control LPS or LTA stimulations were used at 1 μg/mL. CD40, CD80, and CD86 expressions were determined by flow cytometry. Representative dot plots are shown in the left panel, and mean fluorescent intensity values ±SD for three separate experiments are shown in the right bar graph. (b) 5 × 105 BM-DCs were cultured for 24 h in the absence (Unstim.) or the presence of ST11 (at bacteria : DC ratio of 100 : 1), LPS (0.1 μg/mL), or LTA (1 μg/mL), then supernatants were analyzed by multiplex ELISA. Results are mean values ±SD obtained in three independent cultures and expressed in pg/mL of supernatants for each condition.
