Lactobacillus paracasei Reduces Intestinal Inflammation in Adoptive Transfer Mouse Model of Experimental Colitis
Figure 2
ST11 weakly supports Th17 cell differentiation in DCT cells cocultures. (a) BM-DCs were cocultured with sorted naïve CD4+ T cells for 4 days at DC : T cell ratio of 1 : 2.5 in the absence or the presence of TGFβ (5 ng/mL). Then, cells were stimulated, fixed, and intracellularly stained for T helper 1 (Th1 being IFNγ +) or T helper 17 (Th17 being IL-17+) cell quantification by flow cytometry. Representative dot-plots are shown, numbers indicate % of a gated population. Combination of proinflammatory signals and TGFβ are mandatory for Th17 cell differentiation. (b) Bar graphs represent mean % values ±SD of Th1 (CD4+ IFNγ + IL-17− T cells, black-filled bars) and Th17 (CD4+ IFNγ − IL-17+ T cells, unfilled bars) obtained in three independent experiments in the absence (a) or the presence (c) of TGFβ and analyzed by flow cytometry. As proinflammatory signal, either LPS, LTA, or ST11 was used in a dose-dependent manner. Doses are given in ng/mL for LPS and LTA or bacteria: DC ratio for ST11.
