Lactobacillus paracasei Reduces Intestinal Inflammation in Adoptive Transfer Mouse Model of Experimental Colitis
Figure 3
ST11 feeding inhibits colitis development induced by adoptive transfer of naïve T cell in Rag2−/− mice. (a) 6–8 Rag2−/− mice either PBS-fed (black diamonds, right graph) or ST11-fed (red diamonds, middle graph) were adoptively transferred with 5 × 105 sorted naïve cells. Recipient mice were weighted 3 times a week. As negative control, a group of nontransferred Rag2−/− mice was used (empty diamond, left graph). Weight changes (in % of initial body weight) of control or recipient mice ±SD are shown. Average weight in grams ±SD at the enrollment (i) and at day 0 before colitis induction (ii) is given for each group below the graphs. 4 weeks after cell transfer, mice were sacrificed and assessed for histopathology. Inserts are representative H&E stained pictures of colon sections; black bar represents 50 μm (magnification ×20). (b) Colonic MPO was assessed in every single mouse by ELISA and expressed in ng MPO per mg of total colonic proteins. Student's -test with , tendency ; *, , and ** versus PBS-fed mice.
