Lactobacillus paracasei Reduces Intestinal Inflammation in Adoptive Transfer Mouse Model of Experimental Colitis

Figure 4

Phenotypic and functional characteristics of T cells after transfer. 5 × 10⁵ sorted naïve T cells were adoptively transferred into Rag2^−/− mice. (a) At week 4, mesenteric lymph node cell suspensions were made, stained, and analyzed by flow cytometry. CD4⁺ cells repopulated PBS-fed recipient mice (AT) as efficiently as ST11-fed mice (AT and ST11-fed). As control, representative staining obtained with CD4⁺ cells from unmanipulated C57BL/6 wild-type mice (WT) are shown (lower dot plots) because unmanipulated Rag2^−/− mice do not contain CD4⁺ T cells. (b-d) Phenotype of gated CD4⁺ T cells was assessed by FoxP3, CD45RB, CD62L, CD103, and integrin β7 staining as well as (e) IL-17 and IFNγ secretion upon in vitro stimulation. Percentages are indicated in dot plots. Data are representative of two independent experiments made with 3-4 animals each time.