Protection against Autoimmune Diabetes by Silkworm-Produced GFP-Tagged CTB-Insulin Fusion Protein

Table 1

Five primers synthesized for the construction of the fusion proteins.

No.	Sequence	Length
P1	5′-CGGGATCCATGATTAAATTAAAATTTGG-3′; (BamHI)	28 bp
P2	5′-GGGGCCGGGGCCGTTGCAGTAGTTCTCCA-3′; (GPGP)	29 bp
P3	5′-GGGGCCGGGGCCATTTGCCATACTAAT-3′; (GPGP)	27 bp
P4	5′-GGCCCCCGGCCCCGTGAGCAAGGGCGAGGA-3′; (GPGP)	29 bp
P5	5′-TGCTCGAGTTACTTGTACAGCTCGTCC-3′; (XhoI)	27 bp

The italics in P1 and P5 indicate BamHI and XhoI restriction endonuclease sites, respectively. Bold type indicates the linker peptide (GPGP).