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Clinical and Developmental Immunology
Volume 2011, Article ID 915864, 11 pages
http://dx.doi.org/10.1155/2011/915864
Clinical Study

Immune Dysregulation after Cardiothoracic Surgery and Incidental Thymectomy: Maintenance of Regulatory T Cells despite Impaired Thymopoiesis

1Department of Pediatrics, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
2Department of Microbiology, Immunology & Molecular Genetics, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
3Department of Radiology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
4UCLA AIDS Institute, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
5Jonsson Comprehensive Cancer Center, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA

Received 28 January 2011; Revised 30 March 2011; Accepted 18 April 2011

Academic Editor: Stuart Berzins

Copyright © 2011 Nancy J. Halnon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Gating strategy for Treg. Flow cytometry dot plots and pseudodot plot are shown. Cells gated on CD4+ lymphocytes are then gated on CD25+ and FoxP3+ to quantify Treg. CD45RA+ and CD45RA- populations were quantified among Treg. Treg expressing the highest levels of CD25 include the CD45RA- cells while CD45RA+ Treg express intermediate levels of CD25.

  1. Supplementary Material