Suppressors of Cytokine Signaling 3 Expression in Eosinophils: Regulation by PGE2 and Th2 Cytokines
Figure 1
Semiquantitative expression of SOCS3 and SOCS5 genes in peripheral blood purified CD4 T cells and eosinophils. Relative mRNA levels of SOCS3 and SOCS5 in CD4 T cells ((a) and (b)), and eosinophils ((c) and (d)) of healthy controls, asthmatic and NAEB patients were determined by real-time quantitative PCR. Values were normalized with rRNA gene used as an endogen. Black boxes represent the healthy control group (mean ± SD, ), white boxes represent the asthmatic patient group (mean ± SD, ), and lineated boxes represent the NAEB group (mean ± SD, ). Significant differences in the levels of SOCS3 expression in CD4 T cells were obtained for asthmatic and NAEB patients versus the healthy control group and , resp.). In eosinophils, the SOCS3 level was significantly higher in NAEB patients than in healthy controls . In the case of SOCS5, no significant differences were found between the groups. (e) SOCS3 mRNA levels in bronchial biopsies from healthy controls (mean ± SD, ), asthmatics (mean ± SD, ), and NAEB patients (mean ± SD, ). Significant differences in the levels of SOCS3 expression were obtained for asthmatic versus NAEB patients . The results represent relative gene expression, as determined by the ΔΔCT method.
