Suppressors of Cytokine Signaling 3 Expression in Eosinophils: Regulation by PGE₂ and Th2 Cytokines

Figure 3

SOCS3 expression in peripheral blood eosinophils treated with Th2 cytokines and IFN-γ. Purified eosinophils from healthy donors were cultured with 10 ng/mL of IL-4 (a), IL-5 (b), IL-13 (c), or IFN-γ (d) for different periods of time. Dose-response curves were performed with IL-4, IL-5, IL-13, or IFN-γ (e)–(h) at maximal time response; SOCS3 mRNA was measured by real-time quantitative PCR. The results are expressed as a fold induction relative to untreated eosinophils, and significant differences are indicated. Data represent the geometric mean ± SD from four individuals, and each measure was performed in triplicate.

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