Suppressors of Cytokine Signaling 3 Expression in Eosinophils: Regulation by PGE2 and Th2 Cytokines
Figure 5
PGE2 stimulation of SOCS3 mRNA expression. Purified eosinophils from healthy donors were cultured with 10−4 M of PGE2 for up to 120 min (a), and with increasing doses of PGE2 at 120 min (b), and SOCS3 mRNA was measured by real-time quantitative PCR. The results are expressed as a fold induction relative to untreated eosinophils, and significant differences are indicated. Data represent the geometric mean ± SD from four individuals, and each measure was performed in triplicate.
